Vascular Effects of Hydrogen Sulfide

Methods and Protocols
Humana Press Inc.
  • erschienen am 14. Juni 2020
  • Buch
  • |
  • Softcover
  • |
  • X, 221 Seiten
978-1-4939-9530-1 (ISBN)
This book explores techniques for exploring hydrogen sulfide (H2S) and its effects on the vascular system through numerous experimental animal models and vascular preparations. Alterations of vascular H2S generation/signaling may be involved in the pathogenesis of systemic and pulmonary arterial hypertension, ischemic heart disease, ischemic stroke, preeclampsia, and erectile dysfunction, and H2S also serves as an attractive target for pharmacotherapy of cardiovascular diseases, as well as possible effects on cancer, wound healing, and diabetic retinopathy, among other pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Vascular Effects of Hydrogen Sulfide: Methods and Protocols is an ideal aid for scientists working to extend our knowledge in this valuable and wide-ranging field of study.
1st ed. 2019
  • Englisch
  • NY
  • |
  • USA
  • Für Beruf und Forschung
  • 47
  • |
  • 43 farbige Tabellen, 23 s/w Abbildungen, 47 farbige Abbildungen
  • |
  • 43 Tables, color; 47 Illustrations, color; 23 Illustrations, black and white; X, 221 p. 70 illus., 47 illus. in color.
  • Höhe: 25.4 cm
  • |
  • Breite: 17.8 cm
978-1-4939-9530-1 (9781493995301)
weitere Ausgaben werden ermittelt

1. Synthesis, Metabolism, and Signaling Mechanisms of Hydrogen Sulfide: An Overview

Jerzy Beltowski

2. Lanthionine and Other Relevant Sulfur Amino Acid Metabolites: Detection of Prospective Uremic Toxins in Serum by Multiple Reaction Monitoring Tandem Mass Spectrometry

Alessandra F. Perna, Francesca Pane, Nunzio Sepe, Carolina Fontanarosa, Gabriella Pinto, Miriam Zacchia, Francesco Trepiccione, Evgeniya Anishchenko, Diego Ingrosso, Piero Pucci, and Angela Amoresano

3. Analysis of Vascular Hydrogen Sulfide Biosynthesis

Thomas J. Lechuga and Dong-bao Chen

4. Measurement of Protein Persulfidation: Improved Tag-Switch Method

Emilia Kouroussis, Bikash Adhikari, Jasmina Zivanovic, and Milos R. Filipovic

5. ProPerDP: A Protein Persulfide Detection Protocol

Éva Dóka, Elias S.J. Arnér, Edward E. Schmidt, and Péter Nagy

6. Vascular Effects of H2S-Donors: Fluorimetric Detection of H2S Generation and Ion Channel Activation in Human Aortic Smooth Muscle Cells

Alma Martelli, Valentina Citi, and Vincenzo Calderone

7. In Vitro Measurement of H2S-Mediated Vasoactive Responses

Sona Cacanyiova and Andrea Berenyiova

8. In Vivo Measurement of H2S, Polysulfides, and 'SSNO-mix'-Mediated Vasoactive Responses and Evaluation of 10 Hemodynamic Parameters from Rat Arterial Pulse Waveform

Frantisek Kristek, Marian Grman, and Karol Ondrias

9. Simultaneous Measurements of Tension and Free H2S in Mesenteric Arteries

Elise R. Hedegaard, Anna K. Winther, and Ulf Simonsen

10. The Relaxant Mechanisms of Hydrogen Sulfide in Corpus Cavernosum

Fatma Aydinoglu and Nuran Ogulener

11. Pharmacological Tools for the Study of H2S Contribution to Angiogenesis

Lucia Morbidelli, Martina Monti, and Erika Terzuoli

12. Central Administration of H2S Donors for Studying Cardiovascular Effects of H2S in Rats

Marcin Ufnal and Artur Nowinski

13. Colonic Delivery of H2S Donors for Studying Cardiovascular Effects of H2S in Rats

Marcin Ufnal and Tomasz Huc

14. Measurements for Sulfide-Mediated Inhibition of Myeloperoxidase Activity

Dorottya Garai, Zoltán Pálinkás, József Balla, Anthony J. Kettle, and Péter Nagy

15. Vascular Myography to Examine Functional Responses of Isolated Blood Vessels

Joanne Hart

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